Fig. 5
From: Patient-derived response estimates from zero-passage organoids of luminal breast cancer
Patient-specific sensitivity of zero-passage organoids to hormone withdrawal and tamoxifen treatment A–F Upper panels show mean organoid area at six time points after shifted log transformation (n = 22–1407 organoids per patient, time point, and condition; see Methods) for control cultures (gray), cultures with β-estradiol withdrawn (β-est; black), and cultures with β-est withdrawn plus 200 nM 4-hydroxytamoxifen (4-HT; yellow) or 3 µM 4-HT (orange) on day 5. Overlaid are non-linear least-squares curve fits for area growth as a function of volumetric growth rate. Pathologic estimates of positivity for estrogen receptor (ESR1) and progesterone receptor (PGR) are reported as cellular percentages in the inset. Lower panels summarize the best-fit per-day (d−1) volumetric growth rate for each condition with 90% confidence intervals estimated by support plane analysis. *P < 0.05 after Bonferroni correction for multiple-hypothesis testing