Fig. 5

Fibroblasts have a modest impact on macrophages’ ability to inhibit T cell proliferation. A Scheme for macrophage polarization and subsequent fibroblast and T cell coculture. B T cell subtype composition after 3 days in various coculture conditions: (left panel) percentage CD3⁺CD4⁺ T cells (right panel) percentage CD3⁺CD8⁺ T cells. C and D CellTrace was used to label T cells and measure proliferation of T cell subsets after 3 days in various coculture conditions. C Percentage of proliferating CD3⁺CD4⁺ T cells. D Percentage of proliferating CD3⁺CD8⁺ T cells, GraphPad Prism v.9.3.1 was used to perform two-tailed unpaired T-tests and error bars represent standard deviation