Fig. 4

Fibroblasts influence macrophage polarization. A Scheme for macrophage polarization and subsequent fibroblast coculture. B Flow cytometry analysis of CD45 enriched macrophages following 4 days of culture without polarization media. C-E qPCR analysis of CD45 enriched macrophages following 4 days of culture without polarization media. C Pro-inflammatory genes normalized to day 4 (D4) pro-inflammatory macrophages in monoculture. D Anti-inflammatory genes normalized to D4 anti-inflammatory macrophages in monoculture. E Tumor-associated macrophage genes normalized to D4 anti-inflammatory macrophages in monoculture, unpaired t tests used to determine significance (ns P > 0.05, *P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001) and error bars represent standard deviation