Fig. 3

CD105⁺ fibroblasts exhibit robust adipogenesis and secrete proteins related to polarization of macrophages and angiogenesis. A (left panel) Representative brightfield images (10X objective) of CD105 sorted fibroblasts from strain 169 grown in control or adipogenic media for 2 weeks, fixed, and stained with oil red O (ORO) (scale bar = 200 µm). (right panel) Photograph of wells of a 12-well plate imaged in the left panel. B Quantification of ORO staining in sorted fibroblasts grown in adipogenic media by measuring OD (optical density) 492 nm using a plate-reader spectrophotometer, paired t test (P value = 0.0063). C Representative fluorescent images of DNA (Hoechst, blue), ACTA2 (green), and CD105 (red) expression in fibroblasts from 3 different donors (scale bar = 200 µm). D Concentration of each analyte measured in the supernatant of sorted CD105⁺ and CD105⁻ fibroblasts cultured for 4 days, Paired t test. E Correlation between protein and transcript level expression of each analyte in each strain, (CD105⁻ fibroblasts in blue and CD105⁺ fibroblasts in orange) simple linear regression plotted with 95% confidence intervals