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Fig. 2 | Breast Cancer Research

Fig. 2

From: CPT1A/HIF-1α positive feedback loop induced fatty acid oxidation metabolic pathway contributes to the L-ascorbic acid-driven angiogenesis in breast cancer

Fig. 2

CPT1A enhances BC proliferation both in vivo and in vitro. (A) CPT1A knockdown and overexpression BC cell lines were constructed, and the transfection efficiency was verified by Western blot. (B-D) BC cells proliferation ability was tested by CCK8 (B), colony formation (C) and EdU assays (D). Scale bar: 50 μm. (E) The ratio of G2/M%+S% in different groups was quantified by flow cytometry. Quantification of the flow cytometry result (n = 3). (F) Representative fluorescence imaging and morphology of the nude mice xenograft. The down panel represents the analysis of tumor volume and weight. Size of each grid: 1 mm × 1 mm. (n = 5). (G) IHC staining showed Ki67 expression in tumor specimens from xenografts. Scale bar: 50 μm. *P < 0.05 and **P < 0.01

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Breast Cancer Research

ISSN: 1465-542X

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