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Fig. 3 | Breast Cancer Research

Fig. 3

From: Microtubule associated serine/threonine kinase-3 inhibits the malignant phenotype of breast cancer by promoting phosphorylation-mediated ubiquitination degradation of yes-associated protein

Fig. 3

Knockdown of MAST3 significantly promotes the malignant phenotype of breast cancer. (A). Western blot detection of MAST3 transfection efficiency. Recovery of MAST3 expression owing to the table transfection of shMAST3 plasmid in MCF-7. (B–H): MTT (B), colony formation (C, D), matrix gel invasion (E, F), and wound healing (G, H) assays revealed that the proliferation, clone formation, invasion, and migration abilities of tumor cells increased with the decrease in MAST3 expression, MAST3 expression recovery induced the opposite effect. Scale bar: 100 μm. (I–K): In vivo experiments showing that knocking down MAST3 significantly increased the volume and weight of subcutaneous transplanted tumors in nude mice compared to those in the control group, whereas restoring the expression of MAST3 resulted in the opposite effect. Scale bar: 1 cm. (L–M): Knockdown of MAST3 significantly increased the number of tumor cells forming metastatic foci in the tail-vein lung metastasis experiment, whereas restoring the expression of MAST3 resulted in the opposite effect. (Magnification 20×, Scale Bar: 1000 μm; Magnification 400×, Scale Bar: 30 μm). Paired Student t-test, Columns: mean numbers, Bars: SD, each experiment was performed triplicate. *: P < 0.05; * *: P < 0.01; * * *: P < 0.001

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Breast Cancer Research

ISSN: 1465-542X

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