Fig. 2

Overexpression of MAST3 significantly inhibits the proliferation, invasion, and metastasis of breast cancer cells. (A). Western blot was used to detect the expression of MAST3 in different breast cancer cell lines (n = 7) and normal breast cell lines (MCF-10 A). GAPDH was used as an internal reference. (B). Gradient transfection of MAST3 lentivirus-coated plasmid in MDA-MB-468. Western blot detection of MAST3 transfection efficiency. (C–I): MTT assay (C), colony formation assay (D, E), matrix gel invasion assay (F, G) and wound healing assay (H, I) revealed that the proliferation, clone formation, invasion and migration abilities of tumor cells gradually weakened with increasing MAST3 expression. Scale bar: 50 μm. (J–L): After overexpression of MAST3, the volume and weight of subcutaneous transplanted tumors in nude mice decreased significantly compared to those in the control group. Scale bar: 1 cm. (M-N): Overexpression of MAST3 can significantly reduce the number of lung metastases formed by tumor cells through the tail-vein. (Magnification 20×, Scale Bar: 1000 μm; Magnification 400×, Scale Bar: 30 μm). Paired Student t-test, Columns: mean numbers, Bars: SD, each experiment was performed triplicate. *: P < 0.05; * *: P < 0.01; * * *: P < 0.001