Fig. 2

Co-expression of Erbin with HER2, SLC9A3R1 (NHERF1), and EZR (Ezrin). A) Cell type specific marker genes [ERBB2 (HER2), SLC9A3R1 (NHERF1), and EZR (Ezrin)] are expressed as log₂ (normalized UMI counts). B) Spatial distribution of cells identified by unsupervised clustering based on differential gene expression analysis. C) Violin plots illustrating the selected marker gene expression of each cell type [ERBB2 (HER2), SLC9A3R1 (NHERF1), and EZR (Ezrin)]. The center line in each box depicts the median, and the top and bottom edges depict the first/third quartiles. D) Diagram shows separated spots of SLC9A3R1/EZR^high (red) and SLC9A3R1/EZR^LOW (blue) in DCIS. E) Gene Set Enrichment Analysis (GSEA) from visium comparing SLC9A3R1/EZR^high and SLC9A3R1/EZR^LOW in DCIS. F) GSEA enrichment score of apical_junction pathway from SLC9A3R1/EZR^high vs. SLC9A3R1/EZR^LOW in DCIS. G) Diagram of proposed pathway by which HER2 expression could allow interaction between HER2/Erbin and NHERF1/Ezrin complexes. Created with BioRender.com. H) Erbin expression as log₂ (normalized UMI counts). I) Violin plot illustrating Erbin expression in each cell type. J) Pearson correlation coefficient between Erbin and ERBB2 in each cell type. K) Gene Set Enrichment Analysis (GSEA) from visium comparing Erbin^high and Erbin^LOW in DCIS. L) GSEA enrichment score of the hallmark apical_junction pathway comparing Erbin^high and Erbin^LOW DCIS