Fig. 5

Differential Slc11a1 Expression Leads to Enhanced Reactive Oxygen Species (ROS), Ferrous Ion and NETs Content. A RNA sequencing of neutrophils isolated from mouse chemotherapy models identified 1,284 upregulated and 1,011 downregulated genes (n = 3), B, C reactome and GSEA analyses characterized the functional significance of the differentially expressed genes (DEGs). Enrichment analysis highlighted prominent clustering of differentially expressed genes within the ROS, RNS Production in Phagocyte signaling pathway, D a gene heatmap of the ROS, RNS Production in Phagocyte pathway illustrates significant upregulation of Slc11a1 gene expression in the TCb chemotherapy model compared to controls, E q-PCR analysis revealed a significant upregulation of Slc11a1 mRNA in human neutrophils treated with TCb chemotherapy in vitro, F, G western blot assays showed a marked increase in NRAMP1 protein expression in TCb-treated human neutrophils compared to controls, H–J a significant elevation in ferrous ion content was detected in TCb-treated human neutrophils using the FerroOrange probe, K, L measurement of ROS levels using a ROS detection kit showed a pronounced increase in ROS production in human neutrophils exposed to TCb chemotherapy. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001