Fig. 3

ROR1/CD3 TCE induced T cell activation in a ROR1-dependent manner. (A, B) The activation of CD4⁺, CD8⁺ T cells were determined by flow cytometry via analyzing the activation marker CD25 and CD69. (C, D) The proliferation of CD4⁺ and CD8⁺ T cells was measured after co-cultured with a serially diluted ROR1/CD3 antibody for 96 h. The experiment was performed with three tecnological replicates, and validated by another PBMC donor