Fig. 5

Peripheral blood subpopulations of Th1 and Th2 cells in breast cancer patients during one year. The proportion of T cell subpopulations (A) T helper cells 1 (Th1, CD3 + CD4 + CD183 + CD196+), (B) naïve Th1 (CD3 + CD4 + CD183 + CD196 + CD45RO-), (C) memory Th1 (CD3 + CD4 + CD183 + CD196 + CD45RO+), (D) T helper cells 2 (Th2, CD3 + CD4 + CD194 + CD294+), (E) naïve Th2 (CD3 + CD4 + CD194 + CD294 + CD45RO-) and (F) memory Th2 (CD3 + CD4 + CD194 + CD294 + CD45RO+) in relation to the parent cell population was determined quarterly by flow cytometry. The first measurement (baseline, 0 months) was performed after diagnosis and before initiation of therapy. (G) shows the Th1/Th2 ratio over time. Boxes extend from the 10th to the 90th percentiles. Points below and above the whiskers are drawn as individual data points. * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001, therapy group differences were assessed using two-tailed Mann Whitney U test, differences over time within one group were assessed using two-tailed Wilcoxon matched-paired signed rank test