Fig. 6

Lipid analytes with highly unsaturated acyl chains are increased in endocrine resistant breast cancer cells. (a) Top ten upregulated and downregulated lipid analytes with ≥6 double bonds in T47D (top) and UCD4 (bottom) endocrine resistant cells compared to parental cells. Log2 fold changes ± SEM are shown, with species listed on the left. (b) Fold changes in absolute concentrations of lipid analytes grouped by degree of saturation (≥6 double bonds) in T47D (left) and UCD4 (right) endocrine resistant cells compared to parental (Par). One-way ANOVA was applied to compare endocrine resistant to parental cells. Upregulated are indicated by *P < 0.05; **P < 0.01; ***P < 0.0001; ****P < 0.0001. Downregulated are indicated by ▽P < 0.05, ▽▽P < 0.01, ▽▽▽▽P < 0.001. (c) Concentration of long chain (LC), n-6, and n-3 PUFAs in T47D-TamR and -FulvR compared to parental cells, treated with vehicle (Veh, DMSO) or 10 μm TVB2640 for 72 h. Positive control cells were treated with 10 nM promegestone (R5) on day zero. One-way ANOVA compared T47D-TamR and -FulvR, and R- treated cells to Par T47D cells within the Veh and TVB-2640 treated groups. T-tests (green lines) indicate compare treatments ± TVB-2640. *P < 0.05; **P < 0.01; ***P < 0.0001; ****P < 0.0001