Fig. 1

Lipid metabolic pathways are upregulated in endocrine resistant breast cancer cells. a) Principal component analysis (PCA) plots of T47D and UCD4 parental (Par) and endocrine resistant lines based on RNA-sequencing data (triplicate samples). b) Over representation analysis (ORA) of significantly up- and downregulated genes in lipid pathways (q < 0.05, log2 fold change ≥ 2) from T47D and UCD4 endocrine resistant cells using Gene Ontology (GO) Biological Processes gene sets. T47D-TamR cells showed no significantly downregulated lipid pathways. In some cases, gene cohorts were both significantly up- and downregulated. c and d) Heatmaps of significantly up- and downregulated genes from the “Response to Lipid” process, shared between, (c) T47D-TamR and -FulvR cells (upregulated) and T47D-FulvR cells (downregulated), and (d) UCD4-EWD, -TamR, and -FulvR cells (both up- and downregulated)