Fig. 5

Gamma-delta T cells show transcriptional changes in metastatic patients with breast cancer. A UMAP plots of the re-clustered γδ T cells from low-metastatic (left panel) and high-metastatic burden samples (right panel). B Differential composition (estimated by sccomp) of the three gamma-delta cell clusters relative to the whole gamma-delta population between low and high-burden metastatic patients. Data points are samples. The blue boxplot represents the posterior predictive estimates that indicate the model’s descriptive accuracy for the data [37]. C UMAP plots of gamma-delta T cells in low-metastatic (left panel) and high-metastatic samples (right panel), coloured by the relative transcript abundance of the top 9 marker genes. D mIHC panel shows the expression of pan-cytokeratin (red; tumour cells), CD3 (white; T cells), pan-γδ T cell (green), FGFBP2 (yellow), FOXP3 (light blue) and DAPI (dark blue; nuclei) in primary tumour tissues from non-metastatic early breast cancer patients (EBC; top panel) and metastatic breast cancer patients (MBC; bottom panel). The white dotted box marks γδ T cells that co-express FGFBP2 and are shown in magnified images on the right side. E mIHC image quantification showing the total number of γδ T cells per mm² of tissues (left panel) and FGFBP2 + γδ T cells (right panel) from EBC (n = 6) and MBC tumours (n = 4) with p-value (* = 0.0369)