Fig. 2

Integrated analysis of circulating and tumour infiltrating immune cells. A Gene expression changes (log fold-change) between PBMCs and breast tumour tissue across monocyte classic inflammatory cells. Genes are categorised by their significance in FDR (red: significant in both tissues, blue: significant in either tissue, black: not significant). Dashed lines draw thresholds for fold-change = 2.5 for reference, highlighting areas where genes exhibit substantial expression differences between the two conditions across tissues. B upper panel. Healthy/cancer (H/C) gene signature. Boxplots represent the scaled transcript abundance distribution of the cell-type/sample-specific pseudo-bulk data, healthy (blue) and cancer-associated (orange). B lower panel. Validation of the PBMC signature in breast tissue immune cells. C Transcript abundance per cell type (in cancer) for the 12-gene signature differentiating cancer from healthy state