Fig. 1

PBMCs show unique cell composition and phenotype. A PCA plot of the 25 pseudo-bulk transcriptomes, coloured by healthy (blue) and metastatic (orange) conditions. B Differential cell composition between healthy and cancer patients. Dots are coloured by cell type (also described in Fig. 2C). The right side of the plot contains cells that are enriched in cancer patients, while the cells on the left are enriched in healthy donors. The error bars represent the 95% credible interval for the estimates. The significantly enriched cell types are defined by having the 95% credible interval outside the −0.5/0.5 logit-fold-change interval. C Scaled transcription abundance of cell-type-specific pseudo-bulk samples for the top differentially abundant gene transcripts between healthy vs metastatic breast cancer (FDR < 0.01; log fold change > 2). pDC, Plasmacytoid dendritic cells; cDC, Conventional dendritic cells; Tregs, Regulatory T cells; HSPC, Hematopoietic stem and progenitor cells; NK, natural killer; MAIT, Mucosal-associated invariant T cells