Fig. 6

SMYD4 acts by methylating MYH9 protein to promote ubiquitination degradation of MYH9. (A)The localization transformation of βcatenin visualized by IF after rescue treatment by SMYD4 delTPR (B)The TOP/FOP luciferase reporter assay after rescue treatment by SMYD4 delTPR. (C) MYH9 lysine methylation levels after SMYD4 and SMYD4delTPR overexpression detected by immunoprecipitation and western blot. (D) MYH9 lysine methylation levels after SMYD4 knockdown detected by immunoprecipitation and western blot. (E) Changes in MYH9 protein expression levels over time after CHX treatment detected by westernblot between SMYD4 overexpression and control groups. (F) After CQ and MG132 treatments, MYH9 protein expression levels in SMYD4 overexpression and control groups were detected by westernblot. Ubiquitination levels of MG132-treated MYH9 proteins detected by immunoprecipitation and western blot in SMYD4 overexpression group(G) and SMYD4 knockdown group (H).(I) Number of MYH9-bounded CTNNB promoter regions detected by CHIP and real-time qPCR. All experiments were repeat three times (n = 3), compared variances using F test and analyzed using an unpaired t-test, error bars represent SD.*p < 0.05; **p < 0.01;***p < 0.001; ****p < 0.001