Fig. 5

SMYD4 functions through the TPR domain combined with the Ccoil domain of MYH9. (A)Diagrammatic representation of SMYD4 and its truncated forms. 293T cells were transfected with the indicated constructs subjected to immunoprecipitation with anti-HA (against MYH9). The red boxes represents the pull-down bands. (B) Diagrammatic representation of MYH9 and its truncated forms. 293T cells were transfected with the indicated constructs subjected to immunoprecipitation with anti-HIS (against SMYD4). The red boxes represents the pull-down bands. SMYD4 delTPR rescues SMYD4-inhibited cell proliferation and invasion including colony formation assay (C), EdU assay (D), transwell assay (E), scratch assay (F). (G)The expression levels of downstream target genes of Wnt/βcatenin signaling, including MYH9, N-cadherin, Vimentin, C-myc, Cyclin D1 and Survivin detected by western blot. All experiments were repeat three times (n = 3), compared variances using F test and analyzed using an unpaired t-test, error bars represent SD.*p < 0.05; **p < 0.01; ***p < 0.001