Fig. 2

SMYD4 inhibits cell proliferation and invasion. (A) The construction of SMYD4overexpressed CAL-51 cells validated by western blot. The cell proliferation assays and invasion assays were conducted, including, colony formation assay (B), transwell assay (C), EdU assay (D), scratch assay (E) and CCK-8 (F). The apoptotic cells were analyzed through cell cytometry(G). (H)The expression levels of EMTrelated biomarkers, including Ecadherin, Ncadherin, and Vimentin in SMYD4overexpressed CAL-51 cells detected by western blot. (I) The image of tumor volume after in situ injection in SCID mice. (J) The data of tumor volume growth after in situ injection in SCID mice. (K) The protein expression level of SMYD4 in xenograft by IHC. All experiments were repeat three times (n = 3), compared variances using F test and analyzed using an unpaired t-test, error bars represent SD. **p < 0.01;***p < 0.001