Fig. 1

Tumor stemness is elevated in palbociclib-resistant breast cancer. a–f Parental and palbociclib-resistance MCF-7 and T47D cells were cultured in spheroids medium with or without palbociclib for 1 week. a and b Mammosphere formation assay. Representative images of at the left panel and statistical analysis at the right panel. Scale bars represent 100 μm. c and d RT-qPCR showing the expression of stem cell markers Oct-4, Sox2 and Nanog. e and f Flow cytometry showing the proportion of ALDH positive cells. Representative images at the left panel and statistical analysis at the right panel. For a–f, n = 3 biologically independent experiments, means ± s.d. were shown, the p values were calculated by Student’s t-test, ***p < 0.001, **p < 0.01, *p < 0.05