Fig. 5

Cellular neighborhood clustering of NK cells. (A) Cellular neighborhood definition. A circle of 60 μm radius was drawn around each NK cell, identified by either NKp46 or CD56. The cells inside the circle were counted as neighbors of NK cells. (B) All specimens were pooled and analyzed for NK cell neighborhood clusters. The average cellular composition of each of the five NK cell neighborhood clusters is shown. (C) Left: Scatterplots of two specimens with comparatively high NK cell counts to illustrate the distribution of the NK cell neighborhoods clusters. PanCK⁺ cells are shown in grey together with all NK cells, which are colored by the cluster they were assigned to. Right: Corresponding pseudocolored mIHC images of each NK cell neighborhood cluster. Scale bars = 60 μm. (D) The average proportion of each NK cell neighborhood cluster is shown for each patient, except for one patient of cohort 1, in which no NK cells were detected. Specimens are sorted by proportions of cluster 5 and color of specimen ID represents HER2 status (HER2^- in blue, HER2⁺ in red). (E) Negative correlation of the T cell dominant clusters 1 and 2 and the tumor dominant cluster 5. (F) Cellular composition of the NK cell neighborhood clusters for HER2^- and HER2⁺ specimens of both cohorts