Fig. 3
From: Natural killer cells occupy unique spatial neighborhoods in HER2- and HER2+ human breast cancers
Single cell analysis of NK cells results in distinct phenotypes related to the proximity to tumor cells and HER2 status. A) Scatterplot of a specimen with, compared to other analyzed specimens, high density of NK cells, identified by either NKp46 or CD56 (left). Pseudocolored image in higher magnification with NK cells, PanCK+ cells and nuclei (Hematoxylin) with yellow arrows highlighting NK cells in tumor nests; scale bar = 50 μm (right). B-D) Single cell distance analysis: Each NK cell from all specimens was placed into either of the two distance groups, based on the distance threshold (20 μm) of each NK cell from PanCK+ cells. Expression of phenotypic and functional markers of all NK cells, grouped as close (< 20 μm) and far (> 20 μm) from PanCK+ cells (B). Those markers that were significantly different in (B) were split into HER2- specimens (C) and HER2+ specimens (D). One dot represents one specimen and the % is calculated of the sum of ROIs per specimen. Boxes represent Q1-Q3 with the line showing the mean and whiskers the smallest and largest data point within the quartile range