Table 2 Mean, median and frequencies of T cells responses according to HLA-A/B ESR1-specific-restricted peptides in healthy donors and metastatic breast cancer patients. ESR1 amino acid sequences were submitted to two prediction algorithms (NetMHCpan3 and SYPEITHI) and peptides binding prediction to HLA-A/B were classified as strong (+++), moderate (++), weak (+) or null (-)
Mutation | Peptides | Healthy Donors | Patients | |||||
|---|---|---|---|---|---|---|---|---|
Intensity, IFN-y spots/105 cells (median [min-max]) | Frequency (%) | Women | Men | Intensity, IFN-y spots/105 cells (median [min-max]) | Frequency (%) | Women | ||
Wild Type | P0 | 0.00 | 0.00 (0/31) | 0/19 | 0/12 | 28.50 [10.00–84.50] | 16.00 | 4/25 |
D538G | P1 | 0.00 | 0.00 (0/26) | 0/14 | 0/12 | 26.00 [11.50–70.00] | 19.00 | 4/21 |
P2 | 13.50 | 3.84 (1/26) | 1/14 | 0/12 | 60.75 [15.50–106.00] | 9.50 | 2/21 | |
P3 | 29.50 [19.50–39.50] | 8.33 (2/24) | 1/14 | 1/10 | 695.00 | 4.16 | 1/24 | |
P4 | 17.00 | 3.84 (1/26) | 1/15 | 0/11 | 97.50 | 4.00 | 1/25 | |
P5 | 13.00 | 4.34 (1/23) | 1/12 | 0/11 | 12.00 | 4.16 | 1/24 | |
P6 | 0.00 | 0.00 (0/23) | 0/12 | 0/11 | 0.00 | 0.00 | 0/23 | |
Y537S | P7 | 10.00 | 3.70 (0/27) | 1/15 | 0/12 | 116.00 | 4.00 | 1/25 |
P8 | 26.50 | 3.84 (1/26) | 1/15 | 0/11 | 14.75 [13.50–16.00] | 8.30 | 2/24 | |
P9 | 14.00 | 4.16 (1/24) | 1/14 | 0/10 | 106.00 | 4.16 | 1/24 | |
P10 | 15.50 | 3.84 (1/26) | 1/14 | 0/12 | 15.00 [11.00–115.50] | 14.30 | 3/21 | |
P11 | 136.50 | 4.34 (1/23) | 1/11 | 0/12 | 0.00 | 0.00 | 0/23 | |
P12 | 21.50 | 4.34 (1/23) | 1/11 | 0/12 | 0.00 | 0.00 | 0/23 | |
Y537N | P13 | 0.00 | 0.00 (0/22) | 0/11 | 0/11 | 0.00 | 0.00 | 0/24 |
P14 | 0.00 | 0.00 (0/23) | 0/12 | 0/11 | 0.00 | 0.00 | 0/23 | |
P15 | 80.50 | 3.22 (1/31) | 1/19 | 0/12 | 81.50 | 4.00 | 1/25 | |
P16 | 0.00 | 0.00 (1/31) | 0/18 | 0/13 | 65.75 [12.50–119.00] | 9.52 | 2/21 | |
Y537C | P17 | 0.00 | 0.00 (0/27) | 0/15 | 0/12 | 131.50 | 4.00 | 1/25 |
P18 | 0.00 | 0.00 (0/26) | 0/14 | 0/12 | 68.25 [24.50–112.00] | 9.50 | 2/21 | |
P19 | 18.50 | 3.70 (1/27) | 1/15 | 0/12 | 88.50 | 4.00 | 1/25 | |
P20 | 30.50 [20.00–41.00] | 3.84 (0/26) | 2/14 | 0/12 | 695.00 | 4.16 | 1/24 | |
P21 | 0.00 | 0.00 (0/23) | 0/12 | 0/11 | 0.00 | 0.00 | 0/23 | |