Table 1 Characteristics of HLA-A/B ESR1-specific-restricted peptides predicted by immune informatics database. ESR1 amino acid sequences were submitted to two prediction algorithms (NetMHCpan3 and SYPEITHI) and peptides binding prediction to HLA-A/B were classified as strong (+++), moderate (++), weak (+) or null (-)
Peptide | Sequence | Mutation | Binding predictions | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
A1 | A2 | A24 | B7 | |||||||
NetMHCpan3 | SYFPEITHI | NetMHCpan3 | SYFPEITHI | NetMHCpan3 | SYFPEITHI | NetMHCpan3 | SYFPEITHI | |||
P0 | PLYDLLLEM | WT | - | - | ++ | ++ | - | - | - | - |
P1 | VPLYGLLLEM | D538G | - | - | - | - | - | - | +++ | + |
P2 | NVVPLYGLL | D538G | - | - | - | + | - | + | - | - |
P3 | VVPLYGLLL | D538G | - | - | + | + | - | + | - | + |
P4 | PLYGLLLEM | D538G | - | - | + | ++ | - | - | - | - |
P5 | LYGLLLEML | D538G | - | - | - | + | +++ | ++ | - | - |
P6 | KNVVPLYGL | D538G | - | - | - | + | - | - | - | + |
P7 | NVVPLSDLL | Y537S | - | - | + | + | - | + | - | - |
P8 | KNVVPLSDL | Y537S | - | - | - | + | - | - | + | + |
P9 | VPLSDLLLE | Y537S | - | - | - | - | - | - | - | + |
P10 | PLSDLLLEM | Y537S | - | - | ++ | ++ | - | - | - | - |
P11 | LSDLLLEML | Y537S | +++ | + | - | + | - | - | - | - |
P12 | VVPLSDLLL | Y537S | - | - | - | + | + | + | - | + |
P13 | NVVPLNDLL | Y537N | - | - | ++ | + | - | + | - | - |
P14 | PLNDLLLEM | Y537N | - | - | ++ | ++ | - | - | - | - |
P15 | VPLNDLLLE | Y537N | - | - | - | - | - | - | - | + |
P16 | KNVVPLNDL | Y537N | - | - | - | + | - | - | - | + |
P17 | KNVVPLCDL | Y537C | - | - | - | + | - | - | - | + |
P18 | VVPLCDLLL | Y537C | - | - | + | + | ++ | + | - | + |
P19 | LCDLLLEML | Y537C | ++ | + | - | + | - | - | - | - |
P20 | PLCDLLLEM | Y537C | - | - | ++ | ++ | - | - | - | - |
P21 | NVVPLCDLL | Y537C | ++ | - | - | + | - | + | - | - |