Fig. 3
ESR1D538G specific T cells are polyfunctional producing cells. (A) Representative FACS histogram of ESR1D538G (p1) tetramer staining of respectively irrelevant T cells and specific p1 clone (HDB.5). (B) Functional avidity of HDB.5 T cell clone was evaluated after stimulation with a range of the indicated increasing p1 peptide concentrations (p0 was used as an irrelevant peptide). C-E. Representative histograms of ESR1 specific CD8 T clone cytokine production upon p1 peptide unstimulated (medium), stimulated, and PMA/Iono stimulated; IFN-γ (C), TNF-α (D) and IL-2 (E). F. Representative histograms of HDB.5 T cell clone IFN-γ production unstimulated (medium), stimulated with PMA/Iono (positive control) stimulated with p1 or p0 peptides, stimulated with MCF7 mutant pulsed with p1, stimulated with MCF7 mutant E: T 10:1 or MCF7 mutant E: T 20:1. All data are representative of at least 3 independent experiments