Fig. 2

DNA double-strand breaks in normal breast epithelia as a function of the body mass index. A Representative images from neutral comet assays performed on human breast tissue from reduction mammoplasties (premenopausal patients with contrasted BMIs). B Comet Olive tail moment as a function of BMI in samples from premenopausal (N = 36) and postmenopausal (N = 28) patients. r, Pearson’s correlation coefficients. C Illustration of immunodetection of 53BP1 in normal breast tissue samples from the Komen Tissue bank. Arrowheads indicate DNA damage foci. D Association between BMI and 53BP1 foci numbers in normal breast tissue. r, Pearson’s correlation coefficient. E 53BP1 foci in donors with normal weight (N) or obesity (OB). The lines indicate matched comparisons. ** P = 0.002 (paired t-test)