Fig. 1

Effect of diet-induced obesity on DNA damage in the mammary gland. A Weight of C57BL/6 mice after 20 weeks on a control or lard-based high-fat diet. **** P < 0.0001 (t test with Welch’s correction). B 53BP1 damage foci Immunodetection (arrowheads) in the mammary gland from mice fed a control or high-fat diet. C Quantification of 53BP1 damage foci in mammary epithelial cells. **** P < 0.0001 (t test with Welch’s correction). D 53BP1 foci as a function of the serum leptin-to-adiponectin ratio in mice fed control or high-fat lard diets. r, Spearman’s correlation coefficients. E Comet assay on mouse mammary glands. F Quantification of comet Olive tail moment. * P < 0.05 (t test with Welch’s correction). Symbols on the graphs represent different animals