Fig. 1

A flowchart visualization of the study design, with the top (Section 1) showing the annotation, training, and cross-validation of the Convolutional Neural Network (CNN). The bottom (Section 2) illustrates the setup for the analysis of the tumour microenvironment in the 38 ER-positive patients using a model constructed from the entire annotated dataset. In this section we specify how many of whole slide images are from a needle biopsy or a resection, which represents a larger tissue section removed during surgery