Fig. 7

RNA-sequencing reveals DKK1 downregulation uniquely by T6I-29–1 A. Y537S ESR1 MCF7 breast cancer cells were treated for 24 h with 1 µM compound in the presence of 1 nM E2. A) Gene ontology and B) Reactome of T6I-29–1 A. Increasing red color denotes higher significance (smaller P value), with dot size correlating to number of transcripts. GeneRatio refers to number of transcripts changed in T6I-29–1 A treated cells versus genes associated with each term. C) Uniquely and shared differentially expressed transcripts with T6I-29–1 A, Laso, Rad1901, and ICI. T6I-29–1 A uniquely regulates 204 transcripts. D) Pathways most differentially regulated by T6I-29–1 A. E) DKK1 downregulation by different antietrogen treatments. T6I-29–1 A most significantly downregulates the gene expression, followed by ICI