Fig. 3

Elevated cell adhesion proteins are a distinguishing characteristic of cilengitide resistant and sensitive lines. A & B. Bar graphs of summed z-scores ranked from the highest to lowest sum for the cilengitide target integrins ITGAV and ITGB5 from the BR80 (A) and ITGAV, ITGB3, and ITGB5 from the CCLE (B) proteomics datasets are shown. C. Surface-expressed ITGAV:ITGB3 was assessed using flow cytometry and percentages of positive cells were quantified for each cell line, then plotted by sensitivity to cilengitide (left) or by cell line (right). No significant (ns) difference was found (t-test) between the sensitive and resistant lines. The mean with S.E.M. is shown. D. Protein levels of cilengitide targets ITGAV and ITGB3 were probed by immunoblot, with beta-actin (same blot as in Fig. 4F) serving as a loading control. Cell lines were loaded from left to right by their cilengitide sensitivity (most to least resistant). E. Three independent cell protein lysates from each cell line were quantified for ITGAV and ITGB3 and normalized to beta-actin. Resistant lines are colored in red, while sensitive lines are blue. Mean with S.E.M. is shown. F. TNBC-associated proteins (DisGeNET) were assessed for differential protein abundance (p < 0.05, unadjusted Wilcoxon signed rank) in the BR80 dataset between resistant (red) and sensitive (blue) lines and the abundance of the 7 proteins meeting the cutoff is shown as a heatmap with higher abundance in red and lower in blue. G. Differential protein abundance of the resistant and sensitive cell lines was determined across the entire BR80 proteomics dataset and used to perform gene set enrichment analysis (see Methods). The -log of the p-value is plotted for pathways that reached significance (p < 0.01)