Fig. 4
From: Oncogene activated human breast luminal progenitors contribute basally located myoepithelial cells
PIK3CAH1047R-iHBECCD117 luminal progenitors give rise to both lobular and ductal luminal cells in vivo. A Confocal imaging of a representative paraffin section of reduction mammoplasty specimens multicolor-stained for ALDH1A3 (green), CALML5 (white) and K23 (red), and visualized to enhance a combination of white and green (left) and white and red (right), respectively. CALML5 is primarily confined to acini (9 out of 9 biopsies), while ALDH1A3 and K23 are mostly found in extralobular terminal ducts (ETD) (8 out of 10 and 5 out of 9 biopsies, respectively). Arrowheads = acini, arrows = ETDs. Scale bar = 50 µm. B Confocal imaging of paraffin sections of PIK3CAH1047R-iHBECCD117 cells in NOG mice costained for ALDH1A3 (green) and CALML5 (white; left), or keratin K23 (red) and CALML5 (white; right). Note that PIK3CAH1047R-iHBECCD117 expresses both ductal and lobular markers, albeit with a tendency of separation. Scale bar = 50 µm