Fig. 6

Multiplex imaging demonstrates a distinct profile of epithelial and immune cells in AI vs. GI mammary glands. A. Schematic diagram showing the workflow of multiplexing imaging of selected markers in mammary glands of AI and GI mice at different time points. n = 3 per group and the number of fields analyzed is n = 12. B-D. The representative 10 × merged figures (inset 40 × merged) and bar diagrams showing the normalized % of B. CD206 + M2-like macrophages; C. CCL9 + CD206 + M2-like macrophages; and D. ERα expressing ELF5 + LP cells in mammary glands of AI-day 11 vs. GI-day 25 mice. E–H. The representative 10 × merged figures (inset 40 × merged) and bar diagrams showing the normalized E. % of CD206 + M2-like macrophages; F. Wnt5a + ELF5 + ; G. ELF5 + ; and H. ERα + ELF5 + LP cells in mammary glands of AI-day 28 vs. GI-day 28 mice. ns: non-significant, * p-value > 0.05, **p-value > 0.01, *** p-value > 0.001, **** p-value > 0.0001