Fig.3
Expression pattern of acute phase response genes is distinct in AI and GI glands. Quantitative RT-PCR analysis of A. Stat3, B. C/EBPδ, C. CtsL, D. CtsB, E. Orm1, F. Orm2, G. Lrg1, H. Slpi, I. CD14, J. Chi3L1, and K. MMP2. Expression normalized to RPL37. n = 3 per group per time point. Li. Western blot analysis and quantitation of Lii. pStat3Y705, Liii. Stat3, Liv. CtsB, and Lv. CD14. M. Temporal pattern of pSTAT3Y705 positive cell distribution in AI and GI glands between day 7 and day 28 postpartum. n = 6 per group per time point. Ni. Representative images of FFPE sections of AI (day 11 PPM) and GI (day 22 and 25 PPM) mammary glands subjected to immunofluorescence analysis of pStat3Y705 (green) and CD14 (red). DAPI (blue) was used to counterstain the nuclei. Nii. Bar diagram showing the number of pStat3.Y705 and CD14 double-positive cells per field of view (FOV) in mammary gland sections of AI (day 11 PPM) and GI (day 22 and 25 PPM). n = 5 per group per time point. Number of FOV = 10