Fig.2

DNA damage response is heightened in AI glands compared to GI glands. A. Representative images of FFPE sections of AI and GI mammary glands subjected to TUNEL assay at indicated time points (day PPM). Arrows indicating apoptotic cells. B. Kinetics of cell death between days 7 and 28 PPM, assessed by TUNEL staining. n = 6 per group per time point. C. Enrichment plot of HALLMARK_DNA_REPAIR pathway in AI vs. GI glands on day 28 PPM. D. phospho-γH2AX positivity in, GI and AI glands harvested on day 120 PPM and in mammary glands of age-matched virgin mice. n = 4 per group. Ei. Western blot analysis of BCL-xL, BCL-xS and cleaved PARP in AI and GI mammary glands at early phase of involution, quantitated in Eii. BCL-xL, Eiii. BCL-xS and Eiv. 55 kDa cleaved PARP, and Ev. 24 kDa cleaved PARP