Fig. 2

Statistical analysis for heparanase immunohistochemistry comparing different breast cancer subtypes and normal breast tissue. For the breast cancer tissue array and slides stained for heparanase: TNBC (n = 75), ER+/PR+ (n = 18), HER2+ (n = 14), normal (n = 4), and DCIS (n = 5), (A) images were taken of heparanase-stained AMSBIO BR1202B breast cancer tissue array and normal and DCIS slides on an Evos FL Auto 2 microscope (40×). (B) A one-way ANOVA test indicated no significant difference in the H-scores of heparanase-stained cells among subtypes. A Kruskal-Wallis test indicated that there was no significant difference: (C) in the percentage of heparanase positively stained cells in the tissue sections of normal breast tissue, DCIS, and invasive breast cancer subtypes; (D) in the percentage of heparanase weakly stained cells in the tissue sections of normal breast tissue, DCIS, and invasive breast cancer subtypes; (E) in the percentage of heparanase moderately stained cells in the tissue sections of normal breast tissue, DCIS, and invasive breast cancer subtypes; (F) in the percentage of heparanase strongly stained cells in the tissue sections of normal breast tissue, DCIS, and invasive breast cancer subtypes