Fig. 5

GEN inhibited adipocyte-stimulated migration and invasion potential of E0771 cells. (A) Wound healing of E0771 cells cultured in different CM (obtained after co-culture) was monitored under a phase-contrast microscope. (B) Transwell assays were performed to analyze the cell invasion ability of E0771 in the presence or absence of adipocytes, and four doses of GEN were added in the culture medium. (C) Immunofluorescence staining for E-cadherin/N-cadherin (red) and β-actin (green) in upper chamber E0771 cells. Nuclei were stained with DAPI (blue). (D-G) The protein expressions of E-cadherin, N-cadherin and Vimentin were measured by western blotting analysis, and β-actin protein level was used as a loading normal. (H-K) The protein expressions of Wnt3a, β-catenin and c-Myc were measured by western blotting analysis, and β-tubulin protein level was used as a loading norma. All values were expressed as mean ± S.D. (n = 3 in each group). * P < 0.05, ** P < 0.01 vs. Non-coculture group, # P < 0.05, ## P < 0.01 vs. Coculture group