Fig. 7
From: The deubiquitinating enzyme USP11 regulates breast cancer progression by stabilizing PGAM5
USP11 regulates the stability of PGAM5 by removing the ubiquitin chain linked to K48 in breast cancer cells (A) the ubiquitinated K48 chain is selectively cleaved from PGAM5 by USP11, instead of the K63 chain. (B) Introducing stable point mutations that hinder ubiquitination in K48 eliminates the restriction of PGAM5 ubiquitination promoted by USP11. (C) Sh-USP11 increases the ubiquitination level of PGAM5. (D) We replaced lysine with arginine to generate point mutations in candidate sites, and the protein stability of the K191R mutant was significantly increased. (E) And compared with the wild-type PGAM5, the ubiquitination in K191R PGAM5 is reduced