Fig. 4
From: The deubiquitinating enzyme USP11 regulates breast cancer progression by stabilizing PGAM5
There is an interaction relationship between USP11 and PGAM5 (A) The expression of USP11 in tissues affected by breast cancer, revealing a substantial level of expression by qPCR.(B) Positive correlation between the expression of USP11 and PGAM5 in breast cancer tissue. (C) The interaction between USP11 and PGAM5 protein was detected by exogenous co-ip assay (D) The interaction between USP11 and PGAM5 protein was detected by endogenous co-ip assay. (E) Constructing a truncated body of USP11 based on its protein structure. (F) Verify the structural domain of USP11 and PGAM5 bonding through COIP experiments. (G) Western blot was used to detect the inhibitory effects of sh-USP11 on PGAM5, GPX4, and SLC7A11, as well as the recovery of sh-USP11 by OV-PGAM