Fig. 2
From: The deubiquitinating enzyme USP11 regulates breast cancer progression by stabilizing PGAM5
Effects of PGAM5 on proliferation, apoptosis and invasion in breast cancer (A) Detection of PGAM5 expression in 27 pairs of breast cancer tissues and adjacent tissues by qPCR. (B) Detection of PGAM5 expression in breast cancer tissue by immunohistochemistry(The original magnification is respectively 5x ,10x, 20x,. (C) After transfecting NC, sh-PGAM5# 1 and sh-PGAM5# 2 into MCF-7 and MDA-MB-231cells, the expression of PGAM5 in the cells was detected using Western blotting(D) Clone formation experiment were used to detect the effect of sh-PGAM5#1 and sh-PGAM5#2 on growth, respectively. **, P < 0.01. (E) Analyzing the effect of sh-PGAM5#1 and sh-PGAM5#2on apoptosis in MCF-7 and MDA-MB-231 cells through flow cytometry analysis. (F) The effect of sh-PGAM5#1 and sh-PGAM5#2 on the migration of hepatocellular carcinoma Cell invasion was detected by Transwell(stained with crystal violet, original magnification ×200). **, P < 0.01