Fig. 4

In vitro transduction of patient derived IDC tumor samples. (A) The average transduction efficiency of primary human IDC tumors when transduced with DfAd-GFP versus Ad-GFP at MOI 50. n = 5, **p < 0.005 (B) Effect of CAR Expression on transduction efficiency of DfAd-GFP to Ad-GFP of primary human IDC tumors after batching cases with staining intensity 0–2 into “Low” CAR expression and 3 into “High” CAR Expression (n = 3 in Low and n = 2 in High), *p < 0.05, ns = not significant. (C) The transduction efficiency of primary human tumor sample ID 0.029 when transduced with DfAd-GFP versus Ad-GFP at multiple MOI. n = 3. (D) The transduction efficiency of primary human tumor sample ID 0.030 when transduced with DfAd-GFP versus Ad-GFP at multiple MOI. n = 3. Of the five reported tumor samples, 0.029 and 0.030 were the only two samples that yielded sufficient cells to measure transduction efficiency at multiple MOIs. (E) CAR IHC staining of an IDC tumor that shows CAR staining intensity < 1+. (F) CAR IHC staining of an IDC tumor that shows very high CAR staining intensity 3+. Error bars are SEM