Fig. 6

Increased levels of MYC observed upon GNA13 loss is context dependent. A Immunoblot showing the levels of MYC upon ESR1 silencing in MCF-7 sh-Control and sh-GNA13 cells. B Immunoblot showing the levels of MYC upon ESR1 silencing in ZR-75-1 sh-Control and sh-GNA13 cells. C Immunoblot showing estradiol dependent induction of MYC in MCF-7 cells, MCF-7 cells were deprived of estradiol by treating in Charcoal-Stripped FBS media for 72 h, and then stimulated with either 1 nM E2 in presence or absence of 100 nM fulvestrant for 6h. D Immunoblot showing estradiol dependent induction of MYC expression in ZR-75-1 cells, ZR-75-1 cells were deprived of Estradiol by treating in Charcoal-Stripped FBS media for 48 h, and then stimulated with either 1 nM E2 in presence or absence of 100 nM fulvestrant for 6h. E Immunoblot showing the levels of MYC in MCF-7 cells (sh-Control, sh-GNA13-1, shGNA13-2) cells upon estrogen signaling inhibition using 100 nM Fulvestrant for 4h. F Immunoblot showing the levels of MYC in ZR-75-1 cells (sh-Control, sh-GNA13-1, sh-GNA13-2) cells upon estrogen signaling inhibition using 100 nM Fulvestrant for 24h.For immunoblots, results shown are representative of three independent experiments