Fig. 4

RNA sequencing analysis of ER+ breast cancer cells reveal a connection between GNA13 expression and Myc-related signaling pathways. For all experiments, cells were harvested at 80% confluence and processed as described in Experimental Procedures. A RNA sequencing analysis of MCF-7 sh-Control cells and those in which GNA13 was silenced with sh-GNA13-2. Shown are the results of GSEA Hallmark analysis showing the top five pathways up-and downregulated upon GNA13 silencing. B RNA sequencing analysis of T47D expressing either vector or that harboring-GNA13. Shown are the results of GSEA Hallmark analysis showing top five pathways up-and downregulated upon GNA13 overexpression in T47D cells. C Results of GSEA GO analysis showing top pathways upregulated upon GNA13 knockdown in MCF-7 cells from (A). D Results of GSEA GO analysis showing top pathways downregulated upon GNA13 overexpression in T47D cells from (B). E RNA sequencing analysis of MDA-MB-231 expressing either vector or that harboring GNA13. Shown are the results of GSEA Hallmark analysis showing the top five pathways up-and downregulated upon GNA13 overexpression in MDA-MB-231 cells. F RNA sequencing analysis of SKBR3 expressing either vector or that harboring GNA13. Shown are the results of GSEA Hallmark analysis showing top pathways upregulated upon GNA13 overexpression in SKBR3 cells. Pathways highlighted in black represent MYC and related pathways. All pathways represented have nominal p-value < 0.05 and FDR < 0.25. All RNA sequencing experiments were performed in triplicate