Fig. 3

Gα13 negatively impacts soft agar colony formation and in vivo tumorigenesis in ER+ breast cancer cells. A Soft agar colony formation in MCF-7 cells. MCF-7 cells, expressing control shRNA or that targeting GNA13 as indicated, were subject to soft colony formation assay as described in Experimental Procedures. Top: image showing colonies formed 21 days post seeding. Bottom: quantification of the number of colonies formed. B Soft agar colony formation in ZR-75-1 cells. ZR-75-1 cells expressing control shRNA or that targeting GNA13 as indicated, were subject to soft agar colony as in (A). Top: image showing colonies formed 21 days post seeding. Bottom: quantification of the number of colonies formed. C Soft agar colony formation in T47D cells. T47D cells, expressing vector alone or that harboring GNA13 as indicated, were subject to soft agar colony as in (A). Top: image showing colonies formed 21 days post seeding. Bottom: quantification of the number of colonies formed. D Soft agar colony formation assay in MCF-7 knockdown cells following reintroduction of GNA13. MCF-7 GNA13 knockdown cells, expressing vector alone or that harboring GNA13 as indicated, were subject to soft agar colony as in (A). Top: image showing colonies formed 21 days post seeding. Bottom: Quantification of the number of colonies formed. E Top: quantification of weight of tumors at the endpoint of in vivo tumor formation studies. Bottom: images of tumors post excision. For A–D, results shown are pooled data from three independent experiments. Plotted data is presented as mean ± SD, and p-values are denoted as: *, p < 0.05, **, p < 0.01, ***, p < 0.001, and ****, p < 0.0001 or ‘ns’ for ‘not significant’. All colony images are representative of three independent experiments. See Experimental Procedures for details