Fig. 1

Spy1 increases ductal branching. (a) Ratio of ductal progression past the lymph node in 8-week-old inguinal mammary glands from control littermates (Cntl) and MMTV-Spy1 mice (n = 3). (b) Whole mount analysis of inguinal mammary glands from 6-, 8- and 12-week-old littermate control and MMTV-Spy1 mice. Representative images shown in left panels, and quantification of number of branch points per mm (top right panel) and percentage of fat pad filling (bottom right panel) were quantified using ImageJ software analysis. Scale bar = 0.1 mm. (6-week n = 4-, 8- and 12-week n = 3); Errors bars represent SE; Student’s T-test. *p < 0.05, **p < 0.01, ***p < 0.001