Fig. 4

TFAP2A is a target gene of miR-8072 and is associated with unfavorable prognosis in TNBC. A Venn diagram depicting common putative targets of miR-8072 through bioinformatics analysis using the miRWalk, TargetScan, and miRDB databases. B TFAP2A gene alteration across different breast cancer cohorts in the TCGA database. C Comparison of TFAP2A mRNA expression between breast cancer and normal tissues using the GEPIA database. D Comparison of TFAP2A protein levels between breast cancer and normal tissues using the UALCAN database. E Evaluation of TFAP2A protein levels upon modulation of miR-8072 expression in BT-549 cells. F Luciferase reporter assay confirming a direct interaction between miR-8072 and the 3′UTR of TFAP2A. G Analysis of TFAP2A protein expression in different breast cancer subtypes. H Survival analysis indicating a negative correlation between TFAP2A expression and overall survival in basal-like breast cancer