Table 1 Materials used

Antibodies

anti-CD8 antibody for IHC

Cell Signaling

Cat#98941

anti-CD4 antibody for IHC

Cell Signaling

Cat#25229

anti-PC antibody for western blot

Millipore-Sigma

Cat#HPA043922

anti-beta tubulin antibody for western blot

Developmental Studies Hybridoma Bank

Cat#E7

anti-LDH-A antibody for western blot

Cell Signaling

Cat#2012

anti-MCT-1 antibody for western blot

Abcam

Cat#ab93048

anti-HIF1a antibody for western blot

Cell Signaling

Cat#14179

anti-phospho-PDH antibody for western blot

Cell Signaling

Cat#31866

anti-AKT antibody for western blot

Cell Signaling

Cat#9272

anti-phospho-AKT antibody for western blot

Cell Signaling

Cat#4060

anti-CD45 antibody for flow cytometry

BD

Cat#564279

viability stain for flow cytometry

Invitrogen

Cat#L34961

anti-CD44 antibody for flow cytometry

BD

Cat#741227

anti-CD11c antibody for flow cytometry

BD

Cat#564986

anti-CD4 antibody for flow cytometry

BD

Cat#741913

anti-F4/80 antibody for flow cytometry

BioLegend

Cat#123124

anti-Ly6G antibody for flow cytometry

BioLegend

Cat#127633

anti-TIM3 antibody for flow cytometry

BD

Cat#747618

anti-IA/IE antibody for flow cytometry

BioLegend

Cat#107661

anti-CD19 antibody for flow cytometry

BioLegend

Cat#115531

anti-NK1.1 antibody for flow cytometry

BioLegend

Cat#108727

anti-CD11b antibody for flow cytometry

eBioscience

Cat#46-0112-82

anti-CD8a antibody for flow cytometry

BioLegend

Cat#100758

anti-CD69 antibody for flow cytometry

BioLegend

Cat#104510

anti-CD103 antibody for flow cytometry

BioLegend

Cat#121426

anti-CD3 antibody for flow cytometry

BioLegend

Cat#100216

anti-PD-1 antibody for flow cytometry

BioLegend

Cat#135224

anti-Ly6C antibody for flow cytometry

BioLegend

Cat#128055

anti-FOXP3 antibody for flow cytometry

eBioscience

Cat#17-5773-80A

Chemicals, Peptides, and Recombinant Proteins

AZD3965

MedKoo Biosciences

Cat#1448671-31-5

FX-11

MedChemExpress

Cat#HY16214

Syrosingopine

Sigma-Aldrich

Cat#SML-1908

Mir05

Oroboros

Cat#60101-01

Critical Commercial Assays

CellTiter-Glo

Promega

Cat#G7570

Lactate-Glo

Promega

Cat#J5021

Mouse tumor dissociation kit

Miltenyi Biotec

cat#130–096-730

EZNA HP Total RNA kit

Omega Bio-Tek

Cat#R6812

Verso cDNA Synthesis Kit

Thermo Scientific

Cat##AB-1453/B

Maxima SYBR Green/ROX qPCR Mastermix

Thermo Scientific

Cat#K0222

Clariom S Assay HT, mouse

Affymetrix

Cat#902972

Experimental Models: Cell Lines

MWnt

Hursting lab

NA

E0771

Wendt lab

NA

4T1

Wendt lab

NA

D2.A1

Wendt lab

NA

MDA-MB-231

ATCC

CRM-HTB-26

Experimental Models: Organisms/Strains

Mouse: C57BL6/J: wildtype

Jackson labs

Strain id: 000664

Mouse: BALB/c: wildtype

Charles River

Strain id: 028

Mouse: NSG: NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ

University of North Carolina at Chapel Hill Animal Models Core

NSG

Oligonucleotides

See Table S1 for oligonucleotide primer sequences

Recombinant DNA

pLV PC over expression

This manuscript

NA

pLV empty vector

This manuscript

NA

pLKO.1 scram shRNA

Dharmacon

RHS6848

psi-LVRU6H scram shRNA

Genecopoeia

CSHCTR001

pLKO.1 shPC25 shRNA

Dharmacon

RMM3981-201836951

pLKO.1 shPC28 shRNA

Dharmacon

RMM3981-201846492

psi-LVRU6H shPC-B shRNA

Genecopoeia

MSH074157

psi-LVRU6H shPC-C shRNA

Genecopoeia

MSH074157

SMARTvector inducible shPC shRNA

Dharmacon

V3SM11253-234391411

PC promotor luciferase reporter

This manuscript

NA

Software and Algorithms

FlowJo

FlowJo

FlowJo V10.8.1

ImageJ

FIJI

FIJI V2.13.1

Graphpad Prism

Graphpad Software Inc

Graphpad Prism V9.5.1

KMPlotter

Győrffy et al.[33]

NA

GSEA

Subramanian et al., Liberzon et al., and Reimand et al.[30,31,32]

V4.3.2

CIBERSORTX

Newman et al.[28]

NA