Fig. 7

Depletion of PC modulates the tumor immune microenvironment. A (left panel) Representative IHC staining of sections from control and PC-depleted E0771 tumors. (Right panel) Quantification of CD8⁺ and CD4⁺ T cell numbers (n = 3/group). B Experimental summary of the in vivo study. Mice were orthotopically engrafted with control and PC-depleted E0771 cells (5 × 10⁵ cells per mouse) and their primary mammary tumor growth was observed for 10 days at which point AZD3965 was administered. C Fold change of tumor volume in response to AZD3965 (n = 3/group). D Lactate levels in the indicated tumors determined by luminescent assay (n = 3–4/group). E–G (left panels) Representative IHC for CD8, CD4, and Ki67 staining of tumor sections from control and PC-depleted E0771 tumors under control and AZD3965 treated conditions. (Right panel) Quantification of staining CD8⁺ and CD4⁺ T cells, and Ki67⁺ cells (n = 3/group). Statistical significance determined by one-way ANOVA with Tukey's post-hoc test