Fig. 4
From: Hypoxia-mediated repression of pyruvate carboxylase drives immunosuppression
PC expression is reduced by hypoxia. A Comparison of PC expression profiles in publicly available databases among all tissues (n = 5487 normal, 35,806 cancer), breast (n = 475 normal, 5555 cancer), and lung tissues (n = 1016 normal, 2316 cancer). B PC expression in upper and lower quartiles of HIF-1α signaling ssGSEA score in the METABRIC and TCGA breast cancer databases. C PC gene expression determined by qPCR under normoxia (Norm (21% O2)) and hypoxia (Hyp (1% O2)) in sgRNA control and HIF1ɑ knockout A549 cells (n = 3/group). D PC-gene expression determined by qPCR under normoxia (Norm (21% O2)) and hypoxia (Hyp (1% O2)) in E0771, MDA-MB-231 and metM-Wntlung cells (n = 3–4/group). E PC protein levels in E0771 cells after 48 h of culture in normoxia (Norm (21% O2)) and hypoxia (Hyp (1% O2)). F MDA-MB-231 (231) cells transiently transfected with and 4T1 cells stably expressing firefly luciferase under the control of the PC promoter and renilla luciferase under the control of the CMV promoter cultured under normoxic (Norm, 21% O2) and hypoxic (Hyp, (1% O2)) conditions and analyzed using the Dual-Glo Luciferase Assay System (n = 3/group). G 4T1 cells expressing the PC promoter luciferase reporter described in panel E were orthotopically transplanted for 2 weeks and in vivo PC-firefly/CMV-renilla reporter activity was compared to in vitro cultured cells (n = 5/group). Statistical significance was determined by Mann-Whittney U test (A-B) or unpaired student’s t-test (C-E)