Fig. 7
EDI3 knockdown in HCC1954-luc cells reduces ascites formation. A Representative photographs of mice with (left panel) and without (right panel) ascites. Swelling caused by ascites is indicated using arrows. Scale bar represents 1 cm. B Table representing the ratio of mice positive for ascites compared to all mice. C Ascites volume measured in mice. D Quantitative analysis of luminescence signal detected in 100 μl ascites fluid by ex vivo imaging. Data represent results of seven mice per condition. ‘Non-induced’ are mice injected intraperitoneally with HCC1954-luc shEDI3 cells not treated with doxycycline; ‘induced’ are mice injected with doxycycline-treated HCC1954-luc shEDI3 cells and fed a 625 mg/kg doxycycline containing diet (Ssniff) ad libitum for the duration of the experiment. Box plots: horizontal line, median; box, 25th–75th percentiles; whiskers, min to max (*p < 0.05)