Fig. 2

Doxycycline-induced EDI3 silencing influences colony formation, cell adhesion and anoikis resistance. A Representative images (left panel) and corresponding quantification (right panel) of the wound healing assay with HCC1954-luc shNEG and shEDI3 cells treated with 0.1 µg/ml doxycycline. B Representative images (upper panel) and corresponding quantification (lower panel) of colonies (number and size) formed by HCC1954-luc shEDI3 cells treated with 0.01 or 0.1 µg/ml doxycycline at the time of (plated in ± Dox) or after (Dox added 24 h after plating) plating. C Representative pictures (left) of HCC1954-luc shNEG and shEDI3 cells stained with crystal violet after 30 min of adhesion to a fibronectin matrix and quantification (right panel) shown as RFU after 20 and 30 min measured at 570 nm after destaining. D Viability (RFU) of HCC1954-luc shNEG and shEDI3 cells induced with 0.1 µg/ml doxycycline relative to non-induced control cells (0 μg/ml doxycycline) measured 24 h after plating on a poly-HEMA matrix as a measure of anoikis resistance. Scale bars represent 100 μm. Values in graphs represent mean ± SD from at least three independent experiments (*p < 0.05; **p < 0.01). RFU, relative fluorescence units